RegA, an AraC-Like Protein, Is a Global Transcriptional Regulator That Controls Virulence Gene Expression in Citrobacter rodentium
Emily Hart, Ji Yang, Marija Tauschek, Michelle Kelly, Matthew J Wakefield, Gad Frankel, Elizabeth L Hartland, Roy M Robins-Browne
INFECTION AND IMMUNITY | AMER SOC MICROBIOLOGY | Published : 2008
Citrobacter rodentium is an attaching and effacing pathogen which causes transmissible colonic hyperplasia in mice. Infection with C. rodentium serves as a model for infection of humans with enteropathogenic and enterohemorrhagic Escherichia coli. To identify novel colonization factors of C. rodentium, we screened a signature-tagged mutant library of C. rodentium in mice. One noncolonizing mutant had a single transposon insertion in an open reading frame (ORF) which we designated regA because of its homology to genes encoding members of the AraC family of transcriptional regulators. Deletion of regA in C. rodentium resulted in markedly reduced colonization of the mouse intestine. Examination..View full abstract
We thank Rosanna Mundy (Imperial College, London, United Kingdom) for constructing the STM library of C. rodentium, Judyta Praszkier (The University of Melbourne) for the gift of plasmids used in this study, Sau Fung Lee (University of Melbourne) for FAS data, and Danijela Krmek for assisting with mouse experiments.This work was supported by grants from the Australian National Health and Medical Research Council and the Australian Research Council. E. Hart was the recipient of a Melbourne Research Scholarship. M. Tauschek was supported by a Peter Doherty Fellowship of the Australian National Health and Medical Council.