Journal article

Impact of prior immunological exposure on vaccine delivery by Salmonella enterica serovar Typhimurium

Michelle E Gahan, Diane E Webster, Odilia LC Wijburg, Steven L Wesselingh, Richard A Strugnell

VACCINE | ELSEVIER SCI LTD | Published : 2008


Recombinant Salmonella have been employed as vaccine vectors to deliver DNA and protein vaccines of viral, bacterial and parasitic origin. However, the effectiveness of Salmonella delivery may be hampered by prior immunological exposure to Salmonella. We investigated the effects of prior exposure to the Salmonella typhimurium aroAD strain BRD509 on its ability to deliver the non-toxic C fragment of tetanus toxin (TT). BALB/c mice were orally immunised with BRD509 containing the C fragment expression plasmid pTETtac4, C fragment DNA vaccine pAT153/Cfrag or BRD509 alone and, along with age matched un-vaccinated controls, were immunised again 6 months later with BRD509 (pTETtac4). Prior exposur..

View full abstract