Myogel, a novel, basement membrane-rich, extracellular matrix derived from skeletal muscle, is highly adipogenic in vivo and in vitro
KM Abberton, SK Bortolotto, AA Woods, M Findlay, WA Morrison, EW Thompson, A Messina
CELLS TISSUES ORGANS | KARGER | Published : 2008
BACKGROUND/AIMS: Biological and synthetic scaffolds play important roles in tissue engineering and are being developed towards human clinical applications. Based on previous work from our laboratory, we propose that extracellular matrices from skeletal muscle could be developed for adipose tissue engineering. METHODS: Extracellular matrices (Myogels) extracted from skeletal muscle of various species were assessed using biochemical assays including ELISA and Western blotting. Biofunctionality was assessed using an in vitro differentiation assay and a tissue engineering construct model in the rat. RESULTS: Myogels were successfully extracted from mice, rats, pigs and humans. Myogels contained ..View full abstract
Awarded by NHMRC
Awarded by ARC
We wish to thank Effie Keramidaris for her assistance with tissue culture, Warren Flentje for his assistance with ELISA, Xiao-Lian Han for the S100 immunostaining and Dr. Daniel Tilkorn, Dr. Sebastian Tourbach, Liliana Pepe, Sue Mackay and the staff at EMSU for animal surgery. This work was supported by NHMRC grant No.299872 and ARC grant No. DP0557 495.