The Major Surface-Associated Saccharides of Klebsiella pneumoniae Contribute to Host Cell Association
Abigail Clements, Fabien Gaboriaud, Jerome FL Duval, Jacinta L Farn, Adam W Jenney, Trevor Lithgow, Odilia LC Wijburg, Elizabeth L Hartland, Richard A Strugnell
PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2008
Analysing the pathogenic mechanisms of a bacterium requires an understanding of the composition of the bacterial cell surface. The bacterial surface provides the first barrier against innate immune mechanisms as well as mediating attachment to cells/surfaces to resist clearance. We utilised a series of Klebsiella pneumoniae mutants in which the two major polysaccharide layers, capsule and lipopolysaccharide (LPS), were absent or truncated, to investigate the ability of these layers to protect against innate immune mechanisms and to associate with eukaryotic cells. The capsule alone was found to be essential for resistance to complement mediated killing while both capsule and LPS were involve..View full abstract
NHMRC Australian Bacterial Pathogenesis Program Grant 2003 2008, Co-operative Research Centre for Vaccine Technology. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.