Recruitment of adenomatous polyposis coli and beta-catenin to axin-puncta
MC Faux, JL Coates, B Catimel, S Cody, AHA Clayton, MJ Layton, AW Burgess
ONCOGENE | NATURE PUBLISHING GROUP | Published : 2008
The adenomatous polyposis coli (APC) tumour suppressor is a multifunctional protein involved in the regulation of Wnt signalling and cytoskeletal dynamics. Little is known about how APC controls these disparate functions. In this study, we have used APC- and axin-fluorescent fusion proteins to examine the interactions between these proteins and show that the functionally distinct populations of APC are also spatially separate. Axin-RFP forms cytoplasmic punctate structures, similar to endogenous axin puncta. Axin-RFP recruits beta-catenin destruction complex proteins, including APC, beta-catenin, glycogen synthase kinase-3-beta (GSK3-beta) and casein kinase-1-alpha (CK1-alpha). Recruitment i..View full abstract
Awarded by NHMRC program
We thank Dr M Prescott, Dr F Costantini and Dr D Capelluto for cDNA constructs. MCF, MJL and AWB are supported by NH&MRC program grant 234703; AHAC is an NH&MRC RD Wright Research Fellow.