Journal article

Recruitment of adenomatous polyposis coli and beta-catenin to axin-puncta

MC Faux, JL Coates, B Catimel, S Cody, AHA Clayton, MJ Layton, AW Burgess

ONCOGENE | NATURE PUBLISHING GROUP | Published : 2008

Abstract

The adenomatous polyposis coli (APC) tumour suppressor is a multifunctional protein involved in the regulation of Wnt signalling and cytoskeletal dynamics. Little is known about how APC controls these disparate functions. In this study, we have used APC- and axin-fluorescent fusion proteins to examine the interactions between these proteins and show that the functionally distinct populations of APC are also spatially separate. Axin-RFP forms cytoplasmic punctate structures, similar to endogenous axin puncta. Axin-RFP recruits beta-catenin destruction complex proteins, including APC, beta-catenin, glycogen synthase kinase-3-beta (GSK3-beta) and casein kinase-1-alpha (CK1-alpha). Recruitment i..

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