Journal article

IL6/slIL6R complex contributes to emergency granulopoietic responses in G-CSF- and GM-CSF-deficient mice

Francesca Walker, Hui-Hua Zhang, Vance Matthews, Janet Weinstock, Edouard C Nice, Matthias Ernst, Stefan Rose-John, Antony W Burgess

BLOOD | AMER SOC HEMATOLOGY | Published : 2008

Abstract

Mice defective in both granulocyte colony-stimulating factor (G-CSF) and granulocyte-macrophage colony-stimulating factor (GM-CSF) have severely impaired neutrophil production and function, yet these mice respond to acute pathogen challenge with a significant neutrophil response. We have recently reported the development of an in vitro system to detect granulopoietic cytokines secreted from cells isolated from G-CSF, GM-CSF double knockout mice. The conditioned media produced by these cells after stimulation with lipopolysaccharide or Candida albicans supports the production and differentiation of granulocytes (ie, the conditioned media contains neutrophil promoting activity [NPA]). We now s..

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