Expression, purification and characterisation of recombinant Escherichia coli derived chicken interleukin-12
Jesse D Thomas, Kirsten R Morris, Dale I Godfrey, John W Lowenthal, Andrew GD Bean
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | ELSEVIER SCIENCE BV | Published : 2008
Zoonotic viruses, such as H5N1 Avian Influenza, pose major threats to both animals and humans, and with this in mind there is a need for the development of new anti-viral strategies. The cytokine interleukin-12 (IL-12) is known to play a pivotal regulatory role in the anti-viral response due to its role in the induction of the key anti-viral cytokine IFN-gamma. Therefore, strategies which provide a means for the production of therapeutic quantities of IL-12 may be of major benefit. Here we describe the development of biologically active Escherichia coli (E. coli) derived chicken IL-12 (ChIL-12). The single chain ChIL-12 gene was cloned into the pET32b expression vector, transformed into the ..View full abstract
The funding for this work was kindly provided by the Australian Poultry CRC.