Evaluation of splicing efficiency in lymphoblastoid cell lines from patients with splicing-factor retinitis pigmentosa
Lenka Ivings, Katherine V Towns, MA Matin, Charles Taylor, Frederique Ponchel, Richard J Grainger, Rajkumar S Ramesar, David A Mackey, Chris F Inglehearn
MOLECULAR VISION | MOLECULAR VISION | Published : 2008
PURPOSE: Retinitis pigmentosa (RP) is caused by mutations in a variety of genes, most of which have known functions in the retina. However, one of the most perplexing findings of recent retinal genetics research was the discovery of mutations causing dominant RP in four ubiquitously expressed splicing factors. The aim of this study was to use lymphoblast cell lines derived from RP patients to determine whether mutations in two of these splicing factors, PRPF8 and PRPF31, cause measurable deficiencies in pre-mRNA splicing. METHODS: cDNA was prepared from lymphoblastoid cell lines derived from RP patients bearing mutations in the splicing factor genes and controls, grown under a variety of con..View full abstract
Awarded by Wellcome Trust
We acknowledge funding from Wellcome Trust (grant 073988 to C. F. I. and grant 067311 to R. J. G.).