Enrichment of prion protein in exosomes derived from ovine cerebral spinal fluid
Laura J Vella, Deanne LV Greenwood, Roberto Cappai, Jean-Pierre Y Scheerlinck, Andrew F Hill
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | ELSEVIER | Published : 2008
Prion diseases are transmissible neurodegenerative disorders affecting humans and a wide variety of animal species including sheep and cattle. The transmissible agent, the prion, is an abnormally folded form (PrP(Sc)) of the host encoded cellular prion protein (PrP(C)). Distribution of the prion protein in the fluids of species susceptible to these diseases is of importance to human health and the iatrogenic spread of prion disease. Aside from blood which is confirmed to be a source of prion infectivity, it is currently unclear which other body fluids harbor a significant transmission risk. In the current study we examined two ovine fluids; pseudo-afferent lymph and cerebral spinal fluid (CS..View full abstract
We thank Professor John Collinge (MRC Prion Unit) for the gift of monoclonal antibody ICSM18, and Kenneth Goldie (Bio21 Institute) and Anna Freidhuber (Dept. Pathology) for technical assistance with electron microscopy. We also thank Dr. Hung-Hsun Yen for assistance with cerebral spinal fluid collection. This study is supported by an Australian National Health and Medical Research Council (NHMRC) Program Grant (AFH), NHMRC RD Wright Career Development Award (AFH), a University of Melbourne Postgraduate Research Scholarship (LJV) and a Bio21 collaboration support grant.