Journal article

Proliferation is not increased in airway myofibroblasts isolated from asthmatics

JE Ward, T Harris, T Bamford, A Mast, MCF Pain, C Robertson, D Smallwood, T Tran, J Wilson, AG Stewart

EUROPEAN RESPIRATORY JOURNAL | EUROPEAN RESPIRATORY SOC JOURNALS LTD | Published : 2008

Abstract

Airway mesenchymal cells, such as myofibroblasts and airway smooth muscle cells, contribute to inflammation, airway remodelling and hyperresponsiveness in asthma by excessive proliferation and inflammatory mediator production. Using endobronchial biopsies obtained from both nonasthmatic and asthmatic subjects, in situ proliferation was assessed by immunostaining for cyclin D1. The number of immunoreactive cells increased with asthma severity and was restricted to the epithelium and subepithelial connective tissue. Despite increases in smooth muscle area, cyclin D1 was not detected in cells in intact muscle bundles. Biopsy-derived cell cultures were characterised as predominantly myofibroblas..

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