Journal article
The PI(3,5)P2 and PI(4,5)P2 interactomes
B Catimel, C Schieber, M Condron, H Patsiouras, L Connolly, J Catimel, EC Nice, AW Burgess, AB Holmes
Journal of Proteome Research | AMER CHEMICAL SOC | Published : 2008
DOI: 10.1021/pr800540h
Abstract
A comprehensive analysis of the phosphoinositide interactome has been performed using analogues of PI(3,5)P2 and PI(4,5)P2 phosphatidyl phospholipids which were immobilized onto Affi-10 beads or incorporated into liposomes for use as affinity absorbents with cytosolic extracts from colonic carcinoma cell lines. Affinity/LC/MS/MS experiments allowed identification of 388 proteins/protein complexes that appeared to interact specifically with the phosphoinositide targets: a number of novel potential phosphoinositide interacting proteins have been identified. © 2008 American Chemical Society.