Genomic-Bioinformatic Analysis of Transcripts Enriched in the Third-Stage Larva of the Parasitic Nematode Ascaris suum
Cui-Qin Huang, Robin B Gasser, Cinzia Cantacessi, Alasdair J Nisbet, Weiwei Zhong, Paul W Sternberg, Alex Loukas, Jason Mulvenna, Rui-Qing Lin, Ning Chen, Xing-Quan Zhu
PLOS NEGLECTED TROPICAL DISEASES | PUBLIC LIBRARY SCIENCE | Published : 2008
Differential transcription in Ascaris suum was investigated using a genomic-bioinformatic approach. A cDNA archive enriched for molecules in the infective third-stage larva (L3) of A. suum was constructed by suppressive-subtractive hybridization (SSH), and a subset of cDNAs from 3075 clones subjected to microarray analysis using cDNA probes derived from RNA from different developmental stages of A. suum. The cDNAs (n = 498) shown by microarray analysis to be enriched in the L3 were sequenced and subjected to bioinformatic analyses using a semi-automated pipeline (ESTExplorer). Using gene ontology (GO), 235 of these molecules were assigned to 'biological process' (n = 68), 'cellular component..View full abstract
Awarded by National Natural Science Foundation of China
Awarded by International Foundation for Science
Awarded by Scientific and Technological Programme of Guangdong Province
This work was supported by the National Natural Science Foundation of China ( grant no. 36071578), International Foundation for Science ( grant no. B/4018-1), the Scientific and Technological Programme of Guangdong Province ( grant no. 2004B50201020) (XQZ), the Australian Research Council (RBG and AL), the Howard Hughes Medical Institute (PWS), and the National Health and Medical Research Council of Australia ( AL and JM). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.