Journal article
Genomic-bioinformatic analysis of transcripts enriched in the third-stage larva of the parasitic nematode Ascaris suum
CQ Huang, RB Gasser, C Cantacessi, AJ Nisbet, W Zhong, PW Sternberg, A Loukas, J Mulvenna, RQ Lin, N Chen, XQ Zhu
Plos Neglected Tropical Diseases | PUBLIC LIBRARY SCIENCE | Published : 2008
Abstract
Differential transcription in Ascaris suum was investigated using a genomic-bioinformatic approach. A cDNA archive enriched for molecules in the Infective third-stage larva (L3) of A, suum was constructed by suppresive-subtractive hybridization (SSH), and a subset of cDNAs from 3075 dones subjected to microarray analysis using cDNA proves derived in the L3 were sequenced and subjected to bioinformatic analyses using semi-automated pipeline (ESTExplorer). Using gene ontology (GO), 235 of these molecules were assigned to 'biological process' (n = 68), 'cellular component' (n = 50), or 'molecular function' (n = 117). Of the 91 clusters assembled, 56 molecules (61.5%) had homologues/orthologues ..
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Awarded by National Natural Science Foundation of China
Awarded by International Foundation for Science
Awarded by Scientific and Technological Programme of Guangdong Province
Funding Acknowledgements
This work was supported by the National Natural Science Foundation of China ( grant no. 36071578), International Foundation for Science ( grant no. B/4018-1), the Scientific and Technological Programme of Guangdong Province ( grant no. 2004B50201020) (XQZ), the Australian Research Council (RBG and AL), the Howard Hughes Medical Institute (PWS), and the National Health and Medical Research Council of Australia ( AL and JM). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.