Molecular subtyping of feline immunodeficiency virus from cats in Melbourne
D Iwata, SA Holloway
AUSTRALIAN VETERINARY JOURNAL | WILEY | Published : 2008
OBJECTIVE: To determine the subtypes of feline immunodeficiency virus (FIV) present in the domestic cat population in Melbourne. METHODS: Blood samples were collected from 42 cats that had serum antibodies against FIV. DNA was extracted and subjected to polymerase chain reaction (PCR) to amplify variable regions of the envelope (env) and group specific antigen (gag) genes of FIV. PCR products were directly sequenced or sequenced after cloning when direct sequencing yielded ambiguous results. Phylogenetic analysis was performed and comparisons made with representative sequences of different subtypes. RESULTS: The variable region of the env gene was successfully amplified by PCR from 41 of the..View full abstract
We thank Gribbles Pathology Melbourne for providing some of the FIV samples. Funding for this research was provided by The University of Melbourne Veterinary Science Postgraduate Fund.