Molecular and phylogenetic characterization of cytochromes c from Haemonchus contortus and Trichostrongylus vitrinus (Nematoda: Trichostrongylida)
BE Campbell, Aj Nisbet, J Mulvenna, A Loukas, RB Gasser
GENE | ELSEVIER SCIENCE BV | Published : 2008
Although cytochrome c genes (cyt c) and proteins (CYT C) have been relatively well studied in mammals, very little is known about them in parasitic helminths. In the present study, we investigated this group of molecules in Haemonchus contortus (barber's pole worm) and Trichostrongylus vitrinus (black scour worm), two parasitic nematodes of small ruminants. The cyt c gene (512 bp) of H. contortus had one intron and encoded a transcript of 345 nucleotides, whilst that of T. vitrinus (792 bp) had two introns and encoded a transcript of 360 nucleotides. The transcription of cyt c in T. vitrinus was substantially greater in adult males compared with females, although no such gender-enrichment wa..View full abstract
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Awarded by Australian Research Council (ARC)
Thanks to Youssef Abs EL-Cista for confirming the sequence of EST TVm07_A08 and [an Beveridge for comments on the manuscript. Funding from the Australian Research Council (ARC) (LP0667795), Genetic Technologies Limited (GTG) and Meat and Livestock Australia (MLA) is gratefully acknowledged.