Transcriptional Changes in the Hookworm, Ancylostoma caninum, during the Transition from a Free-Living to a Parasitic Larva
Bennett JD Datu, Robin B Gasser, Shivashankar H Nagaraj, Eng K Ong, Peter O'Donoghue, Russell McInnes, Shoba Ranganathan, Alex Loukas
PLOS NEGLECTED TROPICAL DISEASES | PUBLIC LIBRARY SCIENCE | Published : 2008
BACKGROUND: Third-stage larvae (L3) of the canine hookworm, Ancylostoma caninum, undergo arrested development preceding transmission to a host. Many of the mRNAs up-regulated at this stage are likely to encode proteins that facilitate the transition from a free-living to a parasitic larva. The initial phase of mammalian host invasion by A. caninum L3 (herein termed "activation") can be mimicked in vitro by culturing L3 in serum-containing medium. METHODOLOGY/PRINCIPAL FINDINGS: The mRNAs differentially transcribed between activated and non-activated L3 were identified by suppression subtractive hybridisation (SSH). The analysis of these mRNAs on a custom oligonucleotide microarray printed wi..View full abstract
Awarded by Australian Research Council (ARC)
BD was supported by an International Post Graduate Research Award and a University of Queensland Graduate School Scholarship. This research was funded by Australian Research Council (ARC) grants DP0665230 (to RBG and AL) and LP0667795 (to RBG, SR, I. Beveridge and AL). AL is supported by a Senior Research Fellowship from the National Health and Medical Research Council (NHMRC) of Australia. BD also received travel grants from the ARC/NHMRC Research Network for Parasitology and the University of Queensland (UQ GSRTA). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.