Journal article
Role of NADPH oxidase in tissue growth in a tissue engineering chamber in rats
H Hachisuka, GJ Dusting, KM Abberton, WA Morrison, F Jiang
Journal of Tissue Engineering and Regenerative Medicine | JOHN WILEY & SONS LTD | Published : 2008
DOI: 10.1002/term.115
Abstract
Previously we described a subcutaneous arteriovenous loop (AVL)-based tissue engineering chamber system, which contains an intrinsic circulation circuit created by joining the proximal ends of the femoral artery and vein with a venous graft. We showed that nicotinamide adenine dinucleotide phosphate (NADPH) oxidase was involved in mediating neovascularization inside the chamber. However, the role of NADPH oxidase in tissue formation in the chamber is unknown. In this study, we examined the effects of gp91ds-tat, a peptidyl inhibitor of NADPH oxidase, on the growth of engineered tissue blocks, using a rat chamber model. Chambers containing the AVL were filled with Matrigel mixed with gp91ds-t..
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Funding Acknowledgements
The authors thank Jason Palmer and Srinivasa Raju Datla for technical assistance in tissue processing and DHE fluorescence imaging, respectively. This work was funded by project grants from the National Health and Medical Research Council (NHMRC) of Australia, and grants-in-aid from the National Heart Foundation of Australia. G.J.D. also receives a Principal Research Fellowship from NHMRC. The authors declare no conflict of interest.