Journal article

Genetic variants of Malassezia pachydermatis from canine skin: body distribution and phospholipase activity

Claudia Cafarchia, Robin B Gasser, Maria S Latrofa, Antonio Parisi, Bronwyn E Campbell, Domenico Otranto

FEMS YEAST RESEARCH | OXFORD UNIV PRESS | Published : 2008

Abstract

Malassezia pachydermatis isolates (n=185) from skin sites from dogs (n=30) were characterized genetically and biochemically following in vitro culture. Two regions in the chitin synthase-2 gene (chs-2) and the first internal transcribed spacer (ITS-1) of nuclear ribosomal DNA were sequenced, and the phospholipase activity of each isolate was assessed. Three chs-2 (i.e. Ac, Bc and Cc) and eight ITS-1 (i.e. AI1, AI2, AI3, AI4, BI1, CI1, CI2 and CI3) sequence types were defined for all 185 samples. The findings revealed that multiple M. pachydermatis genotypes/subgenotypes could be cultured from healthy dogs or from dogs with single or multiple, generalized skin lesions. Subgenotypes AI1 and BI..

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University of Melbourne Researchers