Journal article

Development of a replicable oriC plasmid for Mycoplasma gallisepticum and Mycoplasma imitans, and gene disruption through homologous recombination in M. gallisepticum

S-W Lee, GF Browning, PF Markham



The genome of Mycoplasma gallisepticum strain R(low) has been sequenced completely, but subsequent genetic studies have been limited by the lack of a replicable vector system. In this study, replicable plasmids were constructed for M. gallisepticum and Mycoplasma imitans using the oriC region upstream from the soj gene. The oriC plasmids of M. gallisepticum (pGTLori) and M. imitans (pMIori) replicated in both species, but Mycoplasma pneumoniae could not support replication of pGTLori. A 180 bp section of the oriC region of M. gallisepticum was found to be the minimal region required for plasmid replication in M. gallisepticum strain S6, the shortest oriC region defined for mycoplasmas. Targe..

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Funding Acknowledgements

This research was funded by grants from the Australian Poultry Cooperative Research Centre (APCRC) to P. F. M. and G. F. B., and S.-W. L. received an APCRC scholarship. S.-W. L. was also in receipt of an International Postgraduate Research Scholarship and a Melbourne International Research Scholarship.