Journal article

Gender-enriched transcripts in Haemonchus contortus - predicted functions and genetic interactions based on comparative analyses with Caenorhabditis elegans

Bronwyn E Campbell, Shivashankar H Nagaraj, Min Hu, Weiwei Zhong, Paul W Sternberg, Eng K Ong, Alex Loukas, Shoba Ranganathan, Ian Beveridge, Russell L McInnes, Gareth W Hutchinson, Robin B Gasser

INTERNATIONAL JOURNAL FOR PARASITOLOGY | ELSEVIER SCI LTD | Published : 2008

Abstract

In the present study, a bioinformatic-microarray approach was employed for the analysis of selected expressed sequence tags (ESTs) from Haemonchus contortus, a key parasitic nematode of small ruminants. Following a bioinformatic analysis of EST data using a semiautomated pipeline, 1885 representative ESTs (rESTs) were selected, to which oligonucleotides (three per EST) were designed and spotted on to a microarray. This microarray was hybridized with cyanine-dye labelled cRNA probes synthesized from RNA from female or male adults of H. contortus. Differential hybridisation was displayed for 301 of the 1885 rESTs ( approximately 16%). Of these, 165 (55%) had significantly greater signal intens..

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