Journal article

In silico analysis of expressed sequence tags from Trichostrongylus vitrinus (Nematoda): comparison of the automated ESTExplorer workflow platform with conventional database searches

Shivashankar H Nagaraj, Robin B Gasser, Alasdair J Nisbet, Shoba Ranganathan

BMC BIOINFORMATICS | BMC | Published : 2008

Abstract

BACKGROUND: The analysis of expressed sequence tags (EST) offers a rapid and cost effective approach to elucidate the transcriptome of an organism, but requires several computational methods for assembly and annotation. Researchers frequently analyse each step manually, which is laborious and time consuming. We have recently developed ESTExplorer, a semi-automated computational workflow system, in order to achieve the rapid analysis of EST datasets. In this study, we evaluated EST data analysis for the parasitic nematode Trichostrongylus vitrinus (order Strongylida) using ESTExplorer, compared with database matching alone. RESULTS: We functionally annotated 1776 ESTs obtained via suppressive..

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University of Melbourne Researchers