Analysis of the genetic diversity within Cryptosporidium hominis and Cryptosporidium parvum from imported and autochtonous cases of human cryptosporidiosis by mutation scanning
Aaron R Jex, Robin B Gasser
ELECTROPHORESIS | WILEY | Published : 2008
The present study investigated sequence variation in part of the 60 kilodalton glycoprotein (pgp60) gene among Cryptosporidium hominis and Cryptosporidium parvum isolates (n=115) from citizens of the UK inferred to have been infected whilst travelling abroad (to 25 countries) or in the UK. The genomic DNA samples from these isolates were subjected to PCR-coupled single-strand conformation polymorphism analysis, followed by targeted sequencing of pgp60. Individual samples were classified to the genotypic and subgenotypic levels based on phylogenetic analysis (Bayesian inference) of pgp60 data, including published sequences for comparison. Based on this analysis, five C. hominis (Ia-If) and fo..View full abstract
Awarded by Melbourne Water Corporation and serves the Australian Research Council
The isolates used in the present study were from the national collection of Cryptosporidium oocysts at the UK Cryptosporidium Reference Unit, NPHS Microbiology Swansea, Singleton Hospital, Swansea, UK. We are gratefiul to Dr. Rachel Chalmers for the provision of samples. Funding support was from Melbourne Water Corporation and serves the Australian Research Council project LP0561862.