Analysis of the genetic diversity within Cryptosporidium hominis and Cryptosporidium parvum from imported and autochtonous cases of human cryptosporidiosis by mutation scanning

AR Jex; RB Gasser

Journal article

Analysis of the genetic diversity within Cryptosporidium hominis and Cryptosporidium parvum from imported and autochtonous cases of human cryptosporidiosis by mutation scanning

AR Jex, RB Gasser

Electrophoresis | WILEY | Published : 2008

DOI: 10.1002/elps.200800422

Abstract

The present study investigated sequence variation in part of the 60 kilodalton glycoprotein (pgp60) gene among Cryptosporidium hominis and Cryptosporidium parvum isolates (n = 115) from citizens of the UK inferred to have been infected whilst travelling abroad (to 25 countries) or in the UK. The genomic DNA samples from these isolates were subjected to PCR-coupled single-strand conformation polymorphism analysis, followed by targeted sequencing of pgp60. Individual samples were classified to the genotypic and subgenotypic levels based on phylogenetic analysis (Bayesian inference) of pgp60 data, including published sequences for comparison. Based on this analysis, five C. hominis (Ia-If) and ..

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University of Melbourne Researchers

Aaron Jex Author

Robin B. Gasser Author

Related Projects (1)

USING MOLECULAR TOOLS TO UNDERSTAND AND CONTROL THE TRANSMISSION OF CRYPTOSPORIDIUM

Cryptosporidium is the major public health concern of water utilities as the parasite has a global impact on the health and survival of mill..

Grants

Awarded by Melbourne Water Corporation and serves the Australian Research Council

Awarded by Australian Research Council

Funding Acknowledgements

The isolates used in the present study were from the national collection of Cryptosporidium oocysts at the UK Cryptosporidium Reference Unit, NPHS Microbiology Swansea, Singleton Hospital, Swansea, UK. We are gratefiul to Dr. Rachel Chalmers for the provision of samples. Funding support was from Melbourne Water Corporation and serves the Australian Research Council project LP0561862.