Adsorption of lysozyme and trypsin by modified MCM-41 with post-synthesis hydrothermal treatment and hydrophobic functionalisation

Abstract

The mesoporous molecular sieve MCM-41 synthesised by the normal hydrothermal method was modified by a post-synthesis hydrothermal treatment and hydrophobically functionalised using hexamethyldisilazane (HMDS) for improving its stability. The MCM-41 samples had hexagonal structures and were used for adsorption of lysozyme and trypsin in MES buffer and the amounts adsorbed were determined by UV-visible spectroscopy. The amounts of lysozyme and trypsin adsorbed onto the HMDS-modified MCM-41 were higher than those on the post-synthesis hydrothermally treated MCM-41, taking into account the reduction in effective pore volume resulting from the HMDS treatment. This shows that the hydrophobicity of..