A role for SNX5 in the regulation of macropinocytosis
Jet Phey Lim, Jack TH Wang, Markus C Kerr, Rohan D Teasdale, Paul A Gleeson
BMC CELL BIOLOGY | BMC | Published : 2008
BACKGROUND: The mechanisms and components that regulate macropinocytosis are poorly understood. Here we have investigated the role of sorting nexin 5 (SNX5) in the regulation of macropinocytic activity. RESULTS: SNX5 is abundantly expressed in macrophages, cells very active in macropinocytosis, and is recruited onto newly-formed macropinosomes. LPS treatment of bone marrow-derived macrophages resulted in a 2.5 fold decrease in macropinosome formation that correlates with a reduction in the levels of SNX5. To investigate the relationship between SNX5 levels and macropinocytic activity we examined the formation of macropinosomes in HEK-FlpIn cells stably expressing GFP-SNX5. Constitutive macro..View full abstract
This work was supported by funding from the National Health and Medical Research Council of Australia. JPL is supported by an Endeavour International Postgraduate Research Scholarship and Melbourne International Research Scholarship. JTHW is supported by an Australian Postgraduate Award and RDT is supported by an NHMRC Senior Research Fellowship. The authors would like to thank Mr Allan Burrows for generously supplying the bone-marrow derived macrophages used in this study and Miss Casey Barnes for assistance quantifying macropinosome-LE/Lysosome fusion.Confocal microscopy was performed at the Australian Cancer Research Foundation (ACRF)/Institute for Molecular Bioscience Dynamic Imaging Facility for Cancer Biology, which was established with the support of the ACRF.