Journal article

Binding, Internalization, and Antigen Presentation of Vaccine-Loaded Nanoengineered Capsules in Blood

Robert De Rose, Alexander N Zelikin, Angus PR Johnston, Amy Sexton, Siow-Feng Chong, Christina Cortez, William Mulholland, Frank Caruso, Stephen J Kent

ADVANCED MATERIALS | WILEY-V C H VERLAG GMBH | Published : 2008

Abstract

The effect of surface chemistry on the binding of LbL (layer-by-layer) capsules to white blood cells (WBCs) in whole human blood was investigated. All capsules were prepared from a fluorescent precursor comprised of PSS/PAH assembled onto silica particles. Sacrificial 1 μm diameter silica particles were used for the sequential deposition of PSS-Rh and then PAH to assemble two bilayers. Four bilayers of PGA/PLL were deposited onto the fluorescent capsule precursor on the silica particles for capsules with an outer layer of PLL or PGA. Monocytes and DCs were identified by first gating on mononuclear cells according to their scatter characteristics and then identifying DCs as lin 1-/HLA-DR and ..

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