Adiponectin, skeletal muscle adiponectin receptor expression and insulin resistance following dexamethasone
Christina Jang, Warrick J Inder, Varuni R Obeyesekere, Frank P Alford
CLINICAL ENDOCRINOLOGY | WILEY | Published : 2008
OBJECTIVE: Skeletal muscle is a major site of adiponectin action and of glucocorticoid-induced insulin resistance. Little human data exist however, regarding the impact of exogenous glucocorticoids on adiponectin receptors in skeletal muscle. DESIGN AND PATIENTS: Twelve subjects with type 2 diabetes and 12 controls underwent blood sampling and muscle biopsy of vastus lateralis before and after 4 days of 4 mg dexamethasone. MEASUREMENTS: (i) Total and high molecular weight (HMW) plasma adiponectin, glucose and insulin; (ii) Skeletal muscle adiponectin receptor AdipoR1 and AdipoR2 mRNA levels by quantitative real time RT-PCR. RESULTS: Baseline total adiponectin (8.0 +/- 0.89 vs. 12.5 +/- 1.46 ..View full abstract
This work was supported by NH & MRC of Australia, St Vincent's Hospital Research Grant, Novo Nordisk Regional Diabetes Support Scheme.