Calcitonin gene-related peptide stimulates mitosis in the tip of the rat gubernaculum in vitro and provides the chemotactic signals to control gubernacular migration during testicular descent
Eric XZ Yong, Jenny Huynh, Pamela Farmer, Sim Y Ong, Magdy Sourial, Susan Donath, Alvin YS Ting, Yan Chow, John M Hutson
JOURNAL OF PEDIATRIC SURGERY | W B SAUNDERS CO-ELSEVIER INC | Published : 2008
BACKGROUND/AIMS: We investigated whether calcitonin gene-related peptide (CGRP) released from sensory genitofemoral nerve branches could stimulate rodent gubernacular growth and provide chemotactic signals for directing inguinoscrotal gubernaculum migration in vitro. MATERIALS AND METHODS: Neonatal rat gubernacula containing a developing cremaster sac (n = 60) were removed at days 0, 2, 4, 6, 8, and 10 (n = 10 per age; n = 5 per experimental group) and placed in organ culture for 24 hours with or without added CGRP (720 nmol/L). The gubernacula were stained for bromodeoxyuridine (BrdU) immunohistochemistry. Cells were counted (3 x 100 cells) in the mesenchymal tip of the gubernaculum to find..View full abstract
This work was supported by the National Health and Medical Research Council of Australia, Canberra, ACT, Australia.