Characterization of agonist-induced endothelium-dependent vasodilatory responses in the vascular bed of the equine digit

Abstract

The role of endothelium-derived relaxing factors was studied in the regulation of vascular responses in the Krebs perfused equine isolated digit. Perfusion pressure was recorded in response to bolus doses of 5-hydroxytryptamine (6 nmol) alone or co-administered with carbachol (CCh; 0.2 μmol), bradykinin (BK; 0.2 nmol), substance P (SP; 0.2 nmol) or sodium nitroprusside (SNP; 0.2 μmol). Nω-Nitro-l-Arginine methyl ester hydrochloride (l-NAME; 300 μm) caused partial but significant inhibition of CCh-induced vasodilatory response, whereas BK and SP-induced responses were resistant to l-NAME. High potassium (K+, 30 mm) and the cytochrome P-450 (CYP) epoxygenase inhibitor, clotrimazole (10 μm) plu..