Journal article
Investigating copper-regulated protein expression in Menkes fibroblasts using antibody microarrays
T Du, SL La Fontaine, M Abdo, SA Bellingham, M Greenough, I Volitakis, RA Cherny, AI Bush, PJ Hudson, J Camakaris, JF Mercer, PJ Crouch, CL Masters, VM Perreau, AR White
Proteomics | WILEY | Published : 2008
Abstract
Neurodegenerative illnesses are characterized by aberrant metabolism of biometals such as copper (Cu), zinc (Zn) and iron (Fe). However, little is known about the metabolic effects associated with altered metal homeostasis. In this study, we used an in vitro model of altered Cu homeostasis to investigate how Cu regulates cellular protein expression. Human fibroblasts containing a natural deletion mutation of the Menkes (MNK) ATP7A Cu transporter (MNK deleted) were compared to fibroblasts overexpressing ATP7A (MNK transfected). Cultures of MNK-transfected (Low-Cu) cells exhibited 95% less intracellular Cu than MNK-deleted (High-Cu) cells. Comparative proteomic analysis of the two cell-lines w..
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