Journal article

Identification of a novel group of putative Arabidopsis thaliana beta-(1,3)-galactosyltransferases

Yongmei Qu, Jack Egelund, Paul R Gilson, Fiona Houghton, Paul A Gleeson, Carolyn J Schultz, Antony Bacic

Plant Molecular Biology | SPRINGER | Published : 2008

Abstract

To begin biochemical and molecular studies on the biosynthesis of the type II arabinogalactan chains on arabinogalactan-proteins (AGPs), we adopted a bioinformatic approach to identify and systematically characterise the putative galactosyltransferases (GalTs) responsible for synthesizing the beta-(1,3)-Gal linkage from CAZy GT-family-31 from Arabidopsis thaliana. These analyses confirmed that 20 members of the GT-31 family contained domains/motifs typical of biochemically characterised beta-(1,3)-GTs from mammalian systems. Microarray data confirm that members of this family are expressed throughout all tissues making them likely candidates for the assembly of the ubiquitously found AGPs. O..

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