Journal article

Differential cytokine expression and regulation of human anti-pig xenogeneic responses by modified porcine dendritic cells

Daniel Stuart Layton, Andrew Gerard Bean, Natalie Michelle Dodge, A David G Strom, Mauro Sergio Sandrin, Francesco Libero Ierino



BACKGROUND: Porcine dendritic cells (DC) are likely to be pivotal cells in the initiation of stimulatory and potential tolerogenic responses to xenoantigens, however, there are limited studies characterizing these antigen presenting cells. METHODS: Porcine PBMC (CD172a(+)) were cultured with GM-CSF and IL-4 and phenotype and functional capabilities assessed. Lipopolysaccharide (LPS), IL-10, and IL-3 were added to the GM-CSF/IL-4 DC cultures to determine phenotypic and functional changes. Quantitative real-time polymerase chain reaction (PCR) for key cytokines was performed and the modified porcine DC were further assessed by primary mixed lymphocyte reaction to determine the effect of LPS, I..

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Funding Acknowledgements

The authors would like to thank Dr Mark Tizard and Dr Jef Hammond for their critical review of this paper and Matt Bruce, Terri O'Neil, Mary Broadway, and Kirsten Morris for their technical support. This work was supported by research Grants from NHMRC, and DSL was a recipient of an Australia Pork Ltd Research Scholarship.