Exon-primed intron-crossing (EPIC) PCR markers of Helicoverpa armigera (Lepidoptera: Noctuidae)
WT Tay, GT Behere, DG Heckel, SF Lee, P Batterham
BULLETIN OF ENTOMOLOGICAL RESEARCH | CAMBRIDGE UNIV PRESS | Published : 2008
Applying microsatellite DNA markers in population genetic studies of the pest moth Helicoverpa armigera is subject to numerous technical problems, such as the high frequency of null alleles, occurrence of size homoplasy, presence of multiple copies of flanking sequence in the genome and the lack of PCR amplification robustness between populations. To overcome these difficulties, we developed exon-primed intron-crossing (EPIC) nuclear DNA markers for H. armigera based on ribosomal protein (Rp) and the Dopa Decarboxylase (DDC) genes and sequenced alleles showing length polymorphisms. Allele length polymorphisms were usually from random indels (insertions or deletions) within introns, although ..View full abstract
This project was supported by The Australian Research Council (ARC) through its funding of the Special Research Centre CESAR (Centre for Environmental Stress and Adaptation Research) and funding from The State Government of Victoria, Australia to WTT. GTB was supported by the Melbourne International Research Scholarship (MIRS) and Melbourne International Fee Remission Scholarship (MIFRS). SFL and WTT were supported by the MaxPlanck-Gesellschaft. Ary Hoffman, Steve McKechnie, Adam Williams, Tamar Stzal and Nancy Endersby provided helpful discussions during the course of this study. Helicoverpa samples were kindly provided by Nancy Endersby, Stephen Cameron, Keshav Kranthi, Yidong Wu and Derek Russell.