Molecular control of the glucan synthase-like protein NaGSL1 and callose synthesis during growth of Nicotiana alata pollen tubes
Lynette Brownfield, Sarah Wilson, Ed Newbigin, Antony Bacic, Steve Read
BIOCHEMICAL JOURNAL | PORTLAND PRESS LTD | Published : 2008
The protein NaGSL1 (Nicotiana alata glucan synthase-like 1) is implicated in the synthesis of callose, the 1,3-beta-glucan that is the major polysaccharide in the walls of N. alata (flowering tobacco) pollen tubes. Here we examine the production, intracellular location and post-translational processing of NaGSL1, and relate each of these to the control of pollen-tube callose synthase (CalS). The 220 kDa NaGSL1 polypeptide is produced after pollen-tube germination and accumulates during pollen-tube growth, as does CalS. A combination of membrane fractionation and immunoelectron microscopy revealed that NaGSL1 was present predominantly in the endoplasmic reticulum and Golgi membranes in younge..View full abstract
This work was supported by the Grains Research and Development Corporation through funding to the Cereal Functional Genomics Group (L.B, S.W. E.N. and A B.) and by a University of Melbourne Research Development Grant awarded to S.M.R. L. B. acknowledges air Australian Post-Graduate Award.