Leishmania adaptor protein-1 subunits are required for normal lysosome traffic, flagellum biogenesis, lipid homeostasis, and adaptation to temperatures encountered in the mammalian host
James E Vince, Dedreia L Tull, Timothy Spurck, Merran C Derby, Geoffrey I McFadden, Paul A Gleeson, Suzanne Gokool, Malcolm J McConville
EUKARYOTIC CELL | AMER SOC MICROBIOLOGY | Published : 2008
The adaptor protein-1 (AP-1) complex is involved in membrane transport between the Golgi apparatus and endosomes. In the protozoan parasite Leishmania mexicana mexicana, the AP-1 mu1 and sigma1 subunits are not required for growth at 27 degrees C but are essential for infectivity in the mammalian host. In this study, we have investigated the function of these AP-1 subunits in order to understand the molecular basis for this loss of virulence. The mu1 and sigma1 subunits were localized to late Golgi and endosome membranes of the major parasite stages. Parasite mutants lacking either AP-1 subunit lacked obvious defects in Golgi structure, endocytosis, or exocytic transport. However, these muta..View full abstract
We thank Keith Gull (University of Oxford) for providing the anti-PRF antibodies.This work was funded by an Australian National Health and Medical Research Council program grant. J.E.V. was supported by an Australian postgraduate award, M.J.M. is an NHMRC principal research fellow, and G.I.M. is an Australian Research Council Federation fellow.