Journal article

Proteomic Characterization of Mouse Cartilage Degradation In Vitro

Richard Wilson, Daniele Belluoccio, Christopher B Little, Amanda J Fosang, John F Bateman

ARTHRITIS AND RHEUMATISM | WILEY-LISS | Published : 2008

Abstract

OBJECTIVE: To develop proteomics to analyze mouse cartilage degradation and correlate transcriptional and translational responses to catabolic stimuli. METHODS: Proteomic techniques were used to analyze catabolism in mouse femoral head cartilage. Using specific methods to prepare cartilage extracts and conditioned media for 2-dimensional polyacrylamide gel electrophoresis and subsequent tandem mass spectrometry, we identified novel proteins and fragments released into the media of control, interleukin-1alpha (IL-1alpha)-treated, and all-trans-retinoic acid (RetA)-treated explants. Fluorescence 2-dimensional difference gel electrophoresis was used to quantify protein expression changes. We al..

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