Journal article

Influence of water potential on nitrification and structure of nitrifying bacterial communities in semiarid soils

Deirdre B Gleeson, Anke M Herrmann, Stephen J Livesley, Daniel V Murphy



The objective of this study was to examine the relationship between soil water potential, nitrifier community structure and nitrification activity in semiarid soils. Soils were collected after a 5-month dry period (end of summer) and subsequently rewetted to specific water potentials and incubated for 7 days prior to analysis of nitrification activity and nitrifier community structure. The approach used in this study targeted a 491bp segment of the amoA gene which encodes the active site of the ammonia monooxygenase enzyme, which is the key enzyme for all aerobic ammonia oxidisers. amoA serves as a useful target for environmental studies since it is both specific and universal for all ammoni..

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Awarded by ARC Linkage

Funding Acknowledgements

This research was conducted with the support of an ARC Linkage grant (LP0560923) in partnership with the Department of Agriculture and Food Western Australia (DAIWA). Support for Dr. AM Herrmann was provided by the European Commission (Marie Curie Outgoing International Fellowship FP6). The authors would like to thank Terry Rose and Michael Smirk for technical assistance.