The Single Mitochondrial Porin of Trypanosoma brucei is the Main Metabolite Transporter in the Outer Mitochondrial Membrane
Mascha Pusnik, Fabien Charriere, Pascal Maser, Ross F Waller, Michael J Dagley, Trevor Lithgow, Andre Schneider
MOLECULAR BIOLOGY AND EVOLUTION | OXFORD UNIV PRESS | Published : 2009
All mitochondria have integral outer membrane proteins with beta-barrel structures including the conserved metabolite transporter VDAC (voltage dependent anion channel) and the conserved protein import channel Tom40. Bioinformatic searches of the Trypanosoma brucei genome for either VDAC or Tom40 identified a single open reading frame, with sequence analysis suggesting that VDACs and Tom40s are ancestrally related and should be grouped into the same protein family: the mitochondrial porins. The single T. brucei mitochondrial porin is essential only under growth conditions that depend on oxidative phosphorylation. Mitochondria isolated from homozygous knockout cells did not produce adenosine-..View full abstract
Awarded by Swiss National Foundation
We thank E. Horn for technical assistance and J. Lukes and P. Englund for providing the antisera. This work was supported by grants 3100-067906 (to A. S.) and PP00A3-114819 (to P. M.) from the Swiss National Foundation and by a grant from the Australian Research Council (to T. L.). M. J. D. is the recipient of an Australian Postgraduate Award.