Journal article

Paired Assessment of Volatile Anesthetic Concentrations with Synaptic Actions Recorded In Vitro

Stuart J McDougall, James H Peters, Lia LaBrant, Xin Wang, Dennis R Koop, Michael C Andresen

PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2008

Abstract

The volatile anesthetic isoflurane poses a number of experimental challenges in the laboratory. Due to its rapid evaporation, the open conditions of most in vitro electrophysiological recording systems make the determination of actual isoflurane concentrations a challenge. Since the absolute anesthetic concentration in solution is directly related to efficacy, concentration measurements are important to allow comparisons between laboratory and clinical studies. In this study we quantify the sources of isoflurane loss during experimentation and describe a method for the measurement of isoflurane concentrations using gas chromatography and mass spectrometry simultaneous to in vitro electrophys..

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Grants

Awarded by National Institutes of Health


Awarded by National Health and Medical Research Council of Australia


Awarded by NATIONAL HEART, LUNG, AND BLOOD INSTITUTE


Funding Acknowledgements

Supported by grants from the National Institutes of Health (HL-058760) and the National Health and Medical Research Council of Australia for which SJM is a C.J. Martin Fellow. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.