Journal article

Oestradiol-induced spermatogenesis requires a functional androgen receptor

Patrick Lim, Charles M Allan, Amanda J Notini, Anna-Maree Axell, Jennifer Spaliviero, Mark Jimenez, Rachel Davey, Julie McManus, Helen E MaClean, Jeffrey D Zajac, David J Handelsman

REPRODUCTION FERTILITY AND DEVELOPMENT | CSIRO PUBLISHING | Published : 2008

Abstract

Spermatogenesis requires androgen but, paradoxically, oestradiol (E2) treatment stimulates spermatogenic development in gonadotrophin- and androgen-deficient hypogonadal (hpg) mice. The mechanisms of E2-induced spermatogenesis were investigated by determining intratesticular E2 levels and testis cell populations in E2-treated hpg male mice, and E2 spermatogenic actions were determined in androgen receptor-knockout (ARKO) mice. Despite increased serum E2 concentrations (150-300 pmol L(-1)), intratesticular E2 concentrations declined fivefold (P < 0.001) in E2-treated v. untreated hpg male mice. Serum FSH reached 40% of normal and total testicular numbers of known FSH-responsive Sertoli, sperm..

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Grants

Funding Acknowledgements

The authors are grateful to Dr W. Alexander (Walter and Eliza Hall Institute, Melbourne, Vic., Australia) for kindly providing the CMV-Cre mice. This research was supported by funding from the National Health and Medical Research Council of Australia. The authors declare that there is no conflict of interest that would prejudice the impartiality of this scientific work.