Journal article

Developing and Testing a Diagnostic Probe for Grape Phylloxera Applicable to Soil Samples

Karen Herbert, Kevin Powell, Alan Mckay, Di Hartley, undefined Herdina, Kathy Ophel-Keller, Michele Schiffer, Ary Hoffmann



Grape phylloxera, Daktulosphaira vitifoliae (Fitch) (Hemiptera Phylloxeridae) is a damaging pest of grapevines (Vitis spp.) around the world, and the management of this pest requires early detection of infestations. Here, we describe the development and validation of a sensitive DNA test for grape phylloxera that can be applied to soil. Species-specific primers were developed for grape phylloxera in the internal transcribed space region 2, and their specificity was confirmed after thorough screening by using a wide range of vineyard organisms and aphid genera. Preliminary testing of the detection limits of the grape phylloxera-specific primers was conducted using field-sourced soil types spi..

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Funding Acknowledgements

We acknowledge the technical assistance of Ginger Korosi (Department of Primary Industries, Rutherglen) and the growers of the King Valley, Nagambie, Rutherglen and Yarra Valley regions for allowing field experiments to be Conducted on personal vineyards. This research was supported by the Commonwealth Cooperative Research Centre Program and conducted through the CRC for Viticulture with Support from Australia's grape growers and winemakers through their investment body the Grape and Wine Research and Development Corporation. Funding also was provided by the Phylloxera and Grape Industry Board of South Australia, the Department of Primary Industries, with matching funds from the Federal Government.