Journal article

DNA damage response protein ASCIZ links base excision repair with immunuglobulin gene conversion

Hayato Oka, Wataru Sakai, Eiichiro Sonoda, Jun Nakamura, Kenjiro Asagoshi, Samuel H Wilson, Masahiko Kobayashi, Kenichi Yamamoto, Joerg Heierhorst, Shunichi Takeda, Yoshihito Taniguchi

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2008

Abstract

ASCIZ (ATMIN) was recently identified as a novel DNA damage response protein. Here we report that ASCIZ-deficient chicken DT40 B lymphocyte lines displayed markedly increased Ig gene conversion rates, whereas overexpression of human ASCIZ reduced Ig gene conversion below wild-type levels. However, neither the efficiency of double-strand break repair nor hypermutation was affected by ASCIZ levels, indicating that ASCIZ does not directly control homologous recombination or formation of abasic sites. Loss of ASCIZ led to mild sensitivity to the base damaging agent methylmethane sulfonate (MMS), yet remarkably, suppressed the dramatic MMS hypersensitivity of polbeta-deficient cells. These data s..

View full abstract