Journal article

Malvolio is a copper transporter in Drosophila melanogaster

Adam Southon, Ashley Farlow, Melanie Norgate, Richard Burke, James Camakaris

JOURNAL OF EXPERIMENTAL BIOLOGY | COMPANY OF BIOLOGISTS LTD | Published : 2008

Abstract

Divalent metal ion transporter 1 (DMT1; also known as SLC11A2) can transport several metals including Fe and Cu in mammalian systems. We set out to determine whether Malvolio (Mvl), the Drosophila melanogaster orthologue of DMT1, can also transport Cu. Overexpression of Mvl caused Cu accumulation in Drosophila S2 cultured cells and conversely dsRNAi knockdown of endogenous Mvl reduced cellular Cu levels. Cell viability under Cu limiting conditions was reduced following dsRNAi knockdown. A homozygous viable Mvl loss-of-function mutant (Mvl97f) was sensitive to excess Cu and female Mvl 97f flies were also sensitive to Cu limitation. An MtnA-EYFP reporter was used as a proxy measure of Cu distr..

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