Journal article

ATP7B expression in human breast epithelial cells is mediated by lactational hormones

Agnes Michaiczyk, Edward Bastow, Mark Greenough, James Camakaris, David Freestone, Philip Taylor, Maria Linder, Julian Mercer, Margaret L Ackland

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | SAGE PUBLICATIONS LTD | Published : 2008

Abstract

A role for the copper transporter, ATP7B, in secretion of copper from the human breast into milk has previously not been reported, although it is known that the murine ortholog of ATP7B facilitates copper secretion in the mouse mammary gland. We show here that ATP7B is expressed in luminal epithelial cells in both the resting and lactating human breast, where it has a perinuclear localization in resting epithelial cells and a diffuse location in lactating tissue. ATP7B protein was present in a different subset of vesicles from those containing milk proteins and did not overlap with Menkes ATPase, ATP-7A, except in the perinuclear region of cells. In the cultured human mammary line, PMC42-LA,..

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