Functional angiotensin II type 2 receptors inhibit growth factor signaling in LNCaP and PC3 prostate cancer cell lines

Abstract

BACKGROUND. There is clear evidence of a tissue-based renin-angiotensin system in the prostate and studies to date suggest that AT1-receptor blocking drugs inhibit the growth of some prostate cancer cell lines and delay the development of prostate cancer. The present studies examine the action of Ang II in two prostate cancer cell lines and report the presence of functional AT2-receptors that regulate the actions of growth factors. METHODS. Immunohistochemistry was used to identify the presence of Ang II and QPCR techniques to examine AT1- and AT2-receptor mRNA expression in androgen-dependent (LNCaP) and independent (PC3) cell lines. The effects of AT1- and AT2-receptor activation upon EGF-..