Journal article

Analysis and characterization of antitumor T-cell response after administration of dendritic cells loaded with allogeneic tumor lysate to metastatic melanoma patients

Nadege Bercovici, Nacilla Haicheur, Severine Massicard, Fredcrique Vernel-Pauillac, Olivier Adotevi, Didier Landais, Isabelle Gorin, Caroline Robert, H Miles Prince, Jean-Jacques Grob, Marie Therese Leccia, Thierry Lesimple, John Wijdenes, Jacques Bartholeyns, Wolf H Fridman, Margarita Salcedo, Estelle Ferries, Eric Tartour

JOURNAL OF IMMUNOTHERAPY | LIPPINCOTT WILLIAMS & WILKINS | Published : 2008

Abstract

The primary goal of cancer vaccines is to induce CD8+ T cells specific for tumor-associated antigens (TAA) but the characterization of these cells has been difficult because of the low sensitivity of ex vivo assays. Here, we focused on TAA-specific CD8+ T-cell responses in melanoma patients after vaccination with autologous dendritic cells loaded with lysates derived from allogeneic tumor-cell lines (Lysate-DC). Out of 40 patients treated, 16 patients developed immune response to tumor-cell lysate and/or CD8+ T cells specific for differentiation and cancer-testis antigens. TAA-specific CD8+ T-cell responses were detected by interferon (IFN)-gamma enzyme-linked immunospot after in vitro sensi..

View full abstract