Fat adaptation followed by carbohydrate restoration increases AMPK activity in skeletal muscle from trained humans
Wee Kian Yeo, Sarah J Lessard, Zhi-Ping Chen, Andrew P Garnham, Louise M Burke, Donato A Rivas, Bruce E Kemp, John A Hawley
JOURNAL OF APPLIED PHYSIOLOGY | AMER PHYSIOLOGICAL SOC | Published : 2008
We have previously reported that 5 days of a high-fat diet followed by 1 day of high-carbohydrate intake (Fat-adapt) increased rates of fat oxidation and decreased rates of muscle glycogenolysis during submaximal cycling compared with consumption of an isoenergetic high-carbohydrate diet (HCHO) for 6 days (Burke et al. J Appl Physiol 89: 2413-2421, 2000; Stellingwerff et al. Am J Physiol Endocrinol Metab 290: E380-E388, 2006). To determine potential mechanisms underlying shifts in substrate selection, eight trained subjects performed Fat-adapt and HCHO. On day 7, subjects performed 1-h cycling at 70% peak O2 uptake. Muscle biopsies were taken immediately before and after exercise. Resting mu..View full abstract
This study was supported by research grants from GlaxoSmithKline (UK) to J. A. Hawley and the National Sports Council of Malaysia (to W. K. Yeo).