The effect of three different miniaturized blood purification devices on plasma cytokine concentration in an ex vivo model of endotoxinemia
M Haase, R Bellomo, I Baldwin, A Haase-Fielitz, M Storr, B Krause, N Boyce, S Svobodova, W Li, SM Bagshaw, S Warrillow, C Langenberg, S Morgera
INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS | SAGE PUBLICATIONS LTD | Published : 2008
PURPOSE: A novel type of adsorptive plasma filtering device (ETX-A) capable of removing endotoxin from blood in a single step has recently been developed using nanotechnology. METHODS: In a miniaturized, ex vivo model of extracorporeal circuits, we tested the capacity to reduce plasma cytokine concentration of ETX-A filters in comparison to standard high-flux (HF) filters, high cut-off (HCO) filters and a control. Blood from six healthy volunteers was spiked with endotoxin and then circulated through closed (ETX-A, control) or open (HF, HCO) circuits. Blood flow was set at 16 ml/min and filtration flow at 1 ml/min. Samples for measurement of IL-1ra and IL-6 were taken at baseline and at 4 ho..View full abstract
Dr Haase holds a postdoctoral Feodor-Lynen Research Fellowship from the Alexander von Humboldt-Foundation, Germany. The Alexander von Humboldt Foundation is a non-profit foundation established by the Federal Republic of Germany for the promotion of international research cooperation.