Journal article
Methylation of the adenomatous polyposis coli (APC) gene in human placenta and hypermethylation in choriocarcinoma cells
NC Wong, B Novakovic, B Weinrich, C Dewi, R Andronikos, M Sibson, F Macrae, R Morley, MD Pertile, JM Craig, R Saffery
Cancer Letters | ELSEVIER IRELAND LTD | Published : 2008
Abstract
Methylation of the human APC gene promoter is associated with several different types of cancers and has also been documented in some pre-cancerous tissues. We have examined the methylation of APC gene promoters in human placenta and choriocarcinoma cells. This revealed a general hypomethylation of the APC-1b promoter and a pattern with monoallelic methylation of the APC-1a promoter in full term placental tissue. However, there was no evidence of a parent-of-origin effect, suggesting random post zygotic origin of methylation. Increased methylation of this promoter was observed in all choriocarcinoma-derived trophoblast cell lines, suggesting a trophoblastic origin of placental APC methylatio..
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Funding Acknowledgements
We would like to thank Dr. Nicole Brooks and Dr. Michael Permezel for help with isolation of placental tissue and parental buccal cells. Professor Lois Salamonson (Prince Henry's Institute of Medical Research, Australia), and Professor Samuel Breit (University of NSW, Australia) for CCA and endometrial cancer cell lines, Steve Nasioulis and Dr. Desiree Du Sart for human blood cells and Drs. Patrick Western and Craig Smith for mouse placental tissue. Special thanks to Shannon Cowie for exon sequencing and protein truncation testing of the APC gene in CCA cell lines. NW is supported by a Philip Desbrow Post Doctoral Fellowship from the Leukaemia Foundation of Australia, RS and JC are supported by National Health and Medical Research Council (Australia) RD Wright Fellowships.