Journal article

Induction of T-cell-mediated skin disease specific for antigen transgenically expressed in keratinocytes

H Azukizawa, H Kosaka, S Sano, WR Heath, I Takahashi, XH Gao, Y Sumikawa, M Okabe, K Yoshikawa, S Itami

EUROPEAN JOURNAL OF IMMUNOLOGY | WILEY-V C H VERLAG GMBH | Published : 2003

Abstract

Transgenic mice were generated to establish an animal model for T-cell-mediated autoimmune skin disease. A membrane-bound form of OVA (mOVA) was specifically expressed under the control of the keratin 5 (K5) promoter in the epidermal and hair follicular keratinocytes of mice. Syngeneic, wild-type mice rejected the skin grafts of K5-mOVA mice with the generation of OVA-specific CTL. To study the CTL response against K5-mOVA skin, we used OT-I transgenic mice, which produce K(b)-restricted, OVA-specific CD8+ T cells. Accelerated rejection of K5-mOVA skin was demonstrated when transplanted onto OT-I mice. Furthermore, OT-I cells, when adoptively transferred into K5-mOVA mice, underwent activati..

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University of Melbourne Researchers