Journal article
Pre-labour Fetal Membranes Overlying the Cervix Display Alterations in Inflammation and NF-κB Signalling Pathways
M Lappas, TL Odumetse, C Riley, NG Reti, SJ Holdsworth-Carson, GE Rice, M Permezel
Placenta | W B SAUNDERS CO LTD | Published : 2008
Abstract
To generate new insights into the process of fetal membrane rupture, we have developed a technique whereby fetal membranes overlying the cervix (i.e. supracervical site, SCS) are tagged in women undergoing term elective Caesarean section. The specific aim is to determine the effect of supracervical apposition on the release of inflammatory mediators and NF-κB signalling in pre-labour fetal membranes. Fetal membranes were collected from both the SCS and from a distal site (DS). The level of NF-κB proteins and its transcriptional co-activator protein CBP and p300 was determined by Western blotting and/or immunohistochemistry (IHC), and cytokine and prostaglandin release was quantified by enzym..
View full abstractRelated Projects (1)
Grants
Awarded by National Health and Medical Research Council
Funding Acknowledgements
Dr. Martha Lappas is in recipient of a National Health and Medical Research Council (NHMRC) RD Wright Fellowship (grant no. 454777). Prof Greg Rice is a NHMRC Principal Research Fellow. The work described in this manuscript was funded by a Melbourne Research Grant Scheme, NHMRC Project grant (grant no. 367615) and ANZ Charitable Trust (Medical Research and Technology Grant). Funding for the Leica Qwin Image Analysis System and the ChemiDoc system was provided by the Medical Research Foundation for Women and Babies. The authors gratefully acknowledge the assistance of the Clinical Research Midwives Valerie Bryant and Sarah Mitchell, the Obstetrics and Midwifery staff of the Mercy Hospital for Women for their co-operation.