Physicochemical Characterization and Stability of Rifampicin Liposome Dry Powder Formulations for Inhalation
Narumon Changsan, Hak-Kim Chan, Frances Separovic, Teerapol Srichana
JOURNAL OF PHARMACEUTICAL SCIENCES | ELSEVIER SCIENCE INC | Published : 2009
Liposomes were used to encapsulate rifampicin (RIF) as an alternative formulation for delivery to the respiratory tract. Factors affecting the stability of liposomes containing RIF were determined. Four liposome suspensions were prepared, containing different millimole ratios of cholesterol (CH) and soybean L-infinity-phosphatidylcholine (SPC) by the chloroform film method, followed by freeze-drying. Cryo-transmission electron microscopy, photon correlation spectroscopy, (2)H and (31)P solid-state nuclear magnetic resonance were used to characterize the liposome suspensions. Differential scanning calorimetry and X-ray diffraction were used to examine the properties of the powder formulations..View full abstract
Awarded by Thailand Research Fund
This work was supported in part by the Thailand Research Fund through the Royal Golden Jubilee PhD program under Grant No. PHD/0119/2546 and also by the National Research Council of Thailand, the Thailand Research Fund, NRCT and NANOTEC center of excellence at Prince of Songkla University, Thailand. Some work was carried out at the electron microscopy unit (EMU) and Faculty of Pharmacy, University of Sydney, Australia. We also thank Dr Herbert Chiou and Dr Patricia Tang for their help and suggestions. We also acknowledge the technical, scientific and financial assistance from the NANO-MNRF for cryo-TEM at the University of Queensland. In addition, we also thank the Bio21 NMR Facility, The University of Melbourne, Australia.